| Título: | Physiological response of Escherichia coli W3110 and BL21 to the aerobic expression of Vitreoscilla hemoglobin |
| Autor(es): | LARA RODRIGUEZ, ALVARO RAUL GALINDO MARTINEZ, JANET JAEN CHAVEZ, KARIM ENRIQUE JUAREZ, MARIANA SIGALA ALANIS, JUAN CARLOS |
| Temas: | Hemoglobina de Vitreoscilla Cultivos aeróbicos E. coli W3110 BL21 Análisis transcripcional |
| Fecha: | 2020 |
| Editorial: | Korea : the Korean Society for Microbiology and Biotechnology (KMB) |
| Citation: | J. Microbiol. Biotechnol. 30(10) 2020 |
| Resumen: | The aerobic growth and metabolic performance of Escherichia coli strains BL21 and W3110 were studied when the Vitreoscilla hemoglobin (VHb) was constitutively expressed in the chromosome. When VHb was expressed, acetate production decreased in both strains and was nearly eliminated in BL21. Transcriptional levels of the glyoxylate shunt genes decreased in both strains when VHb was expressed. However, higher transcription of the α-ketoglutarate dehydrogenase genes were observed for W3110, while for BL21 transcription levels decreased. VHb expression reduced the transcription of the cytochrome bo3 genes only in BL21. These results are useful for better selecting a production host. |
| URI: | http://ilitia.cua.uam.mx:8080/jspui/handle/123456789/768 |
| Aparece en las colecciones: | Artículos |
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| Physiological response of Escherichia coli W3110 and BL21 to the aerobic expression of Vitreoscilla hemoglobin.pdf | 1.32 MB | Adobe PDF | Visualizar/Abrir |
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