DC Field | Value | Language |
dc.contributor.author | DE LA CRUZ HERNANDEZ, MITZI | - |
dc.contributor.author | RAMIREZ CAMPOS, ELISA ALEJANDRA | - |
dc.contributor.author | SIGALA ALANIS, JUAN CARLOS | - |
dc.contributor.author | UTRILLA CARRERI, JOSE | - |
dc.contributor.author | LARA RODRIGUEZ, ALVARO RAUL | - |
dc.coverage.spatial | <dc:creator id="info:eu-repo/dai/mx/cvu/103807">JUAN CARLOS SIGALA ALANIS</dc:creator> | - |
dc.coverage.spatial | <dc:creator id="info:eu-repo/dai/mx/cvu/165121">JOSE UTRILLA CARRERI</dc:creator> | - |
dc.coverage.spatial | <dc:creator id="info:eu-repo/dai/mx/cvu/40351">ALVARO RAUL LARA RODRIGUEZ</dc:creator> | - |
dc.coverage.temporal | <dc:subject>info:eu-repo/classification/cti/7</dc:subject> | - |
dc.date.accessioned | 2021-05-07T19:32:59Z | - |
dc.date.available | 2021-05-07T19:32:59Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Microorganisms, 8, (9) 1444, 2020 | en_US |
dc.identifier.uri | http://ilitia.cua.uam.mx:8080/jspui/handle/123456789/769 | - |
dc.description.abstract | The design of optimal cell factories requires engineering resource allocation for maximizing
product synthesis. A recently developed method to maximize the saving in cell resources released
0.5% of the proteome of Escherichia coli by deleting only three transcription factors. We assessed the
capacity for plasmid DNA (pDNA) production in the proteome-reduced strain in a mineral medium,
lysogeny, and terrific broths. In all three cases, the pDNA yield from biomass was between 33 and
53% higher in the proteome-reduced than in its wild type strain. When cultured in fed-batch mode in
shake-flask, the proteome-reduced strain produced 74.8 mg L−1 pDNA, which was four times greater
than its wild-type strain. Nevertheless, the pDNA supercoiled fraction was less than 60% in all
cases. Deletion of recA increased the pDNA yields in the wild type, but not in the proteome-reduced
strain. Furthermore, recA mutants produced a higher fraction of supercoiled pDNA, compared to
their parents. These results show that the novel proteome reduction approach is a promising starting
point for the design of improved pDNA production hosts. | en_US |
dc.description.sponsorship | MDPI | en_US |
dc.language.iso | Inglés | en_US |
dc.publisher | Suiza : MDPI | en_US |
dc.relation.haspart | 2076-2607 | - |
dc.rights | https://doi.org/10.3390/microorganisms8091444 | - |
dc.rights | https://www.mdpi.com/2076-2607/8/9/1444 | - |
dc.subject | Celdas mínimas | en_US |
dc.subject | Reducción de proteoma | en_US |
dc.subject | Proteoma magro | en_US |
dc.subject | ADN plasmídico | en_US |
dc.title | Plasmid DNA production in proteome-reduced Escherichia coli | en_US |
dc.type | Artículo | en_US |
Aparece en las colecciones: | Artículos
|